Recombinant Human NDUFS1 protein, His & T7-tagged

• Cat.No.: NDUFS1-1716H
• Product Overview: Recombinant Human NDUFS1 aa. (Ala524~Cys727 (Accession # P28331)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.9kDa
• Protein length: Ala524~Cys727 (Accession # P28331)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5

Gene Information

• Gene Name: NDUFS1 NADH:ubiquinone oxidoreductase core subunit S1 [ Homo sapiens (human) ]
• Official Symbol: NDUFS1
• Synonyms: NDUFS1; NADH:ubiquinone oxidoreductase core subunit S1; CI-75k; CI-75Kd; PRO1304; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
• Gene ID: 4719
• mRNA Refseq: NM_001199981.1
• Protein Refseq: NP_001186910.1
• UniProt ID: P28331