Recombinant Human NECTIN2 protein, Fc & Avi-tagged, Biotinylated

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Recombinant Human NECTIN2 protein, Fc & Avi-tagged, Biotinylated

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Cat.No. : NECTIN2-0632H
Product Overview : Biotinylated Human Nectin-2, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description : Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
Source : HEK293
Species : Human
Tag : Fc & Avi
Predicted N Terminal : Gln 32
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 64.0 kDa. As a result of glycosylation, the protein migrates as 66 KDa under reducing (R) condition, and 130 KDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Gln 32 - Leu 360
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : Powder,RT
SDS-PAGE :

Biotinylated Human Nectin-2, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : NECTIN2 nectin cell adhesion molecule 2 [ Homo sapiens (human) ]
Official Symbol : NECTIN2
Synonyms : HVEB; PRR2; CD112; PVRL2; PVRR2; herpesvirus entry protein B; poliovirus receptor-like 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); nectin-2
Gene ID : 5819
mRNA Refseq : NM_001042724.1
Protein Refseq : NP_001036189.1
UniProt ID : Q92692

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