Recombinant Human NECTIN4 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NECTIN4-931HB
• Product Overview: Biotinylated Recombinant Human NECTIN4 protein(Q96NY8-1)(Gly32-Leu146), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly32-Leu146
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.