Recombinant Full Length Human NECTIN4 Protein, C-Flag-tagged

• Cat.No.: NECTIN4-1064HFL
• Product Overview: Recombinant Full Length Human NECTIN4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 55.3 kDa
• AA Sequence: MPLSLGAEMWGPEAWLLLLLLLASF TGRCPAGELETSDVVTVVLGQDAKL PCFYRGDSGEQVGQVAWARV DAGEGAQELALLHSKYGLHVSPAYE GRVEQPPPPRNPLDGSVLLRNAVQA DEGEYECRVSTFPAGSFQAR LRLRVLVPPLPSLNPGPALEEGQGL TLAASCTAEGSPAPSVTWDTEVKGT TSSRSFKHSRSAAVTSEFHL VPSRSMNGQPLTCVVSHPGLLQDQR ITHILHVSFLAEASVRGLEDQNLWH IGREGAMLKCLSEGQPPPSY NWTRLDGPLPSGVRVDGDTLGFPPL TTEHSGIYVCHVSNEFSSRDSQVTV DVLDPQEDSGKQVDLVSASV VVVGVIAALLFCLLVVVVVLMSRYH RRKAQQMTQKYEEELTLTRENSIRR LHSHHTDPRSQPEESVGLRA EGHPDSLKDNSSCSVMSEEPEGRSY STLTTVREIETQTELLSPGSGRAEE EEDQDEGIKQAMNHFVQENG
  TLRAKPTGNGIYINGRGHLVTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adherens junction

Gene Information

• Gene Name: NECTIN4 nectin cell adhesion molecule 4 [ Homo sapiens (human) ]
• Official Symbol: NECTIN4
• Synonyms: LNIR; PRR4; EDSS1; PVRL4; nectin-4
• Gene ID: 81607
• mRNA Refseq: NM_030916.3
• Protein Refseq: NP_112178.2
• MIM: 609607
• UniProt ID: Q96NY8