| Description : |
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. |
| Source : |
E.coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Filtered White lyophilized (freeze-dried) powder. |
| Molecular Mass : |
62.5kDa |
| AA Sequence : |
MKHHHHHHASSFSYEPYYSTSYKRR YVETPRVHISSVRSGYSTARSAYSS YSAPVSSSLSVRRSYSSSSGSLMPS LENLDLSQVAAISNDLKSIRTQEKA QLQDLNDRFASFIERVHELEQQNKV LEAELLVLRQKHSEPSRFRALYEQE IRDLRLAAEDATNEKQALQGEREGL EETLRNLQARYEEEVLSREDAEGRL MEARKGADEAALARAELEKRIDSLM DEISFLKKVHEEEIAELQAQIQYAQ ISVEMDVTKPDLSAALKDIRAQYEK LAAKNMQNAEEWFKSRFTVLTESAA KNTDAVRAAKDEVSESRRLLKAKTL EIEACRGMNEALEKQLQELEDKQNA DISAMQDTINKLENELRTTKSEMAR YLKEYQDLLNVKMALDIEIAAYRKL LEGEETRLSFTSVGSITSGYSQSSQ VFGRSAYGGLQTSSYLMSTRSFPSY YTSHVQEEQIEVEETIEAAKAEEAK DEPPSEGEAEEEEKDKEEAEEEEAA EEEEAAKEESEEAKEEEEGGEGEEG EETKEAEEEEKKVEGAGEEQAAKKK D. |
| Purity : |
Greater than 90.0% as determined by SDS-PAGE. |
| Stability : |
Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 centigrade for a limited period of time; it does not show any change after two weeks at 4 centigrade. |
| Storage : |
Store lyophilized protein at -20 centigrade. |
| Storage Buffer : |
NEFL filtered (0.4 µm) and lyophilized from 0.5 mg/mL solution in 85 mM, 15 mM Tris and Glycine, pH 8.5. |
| Dilutions : |
It is recommended to add 200 µL of deionized waterto prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. |
| Shipping : |
Shipped at Room temp. |
