Recombinant Human NEFL Protein, His-tagged

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Recombinant Human NEFL Protein, His-tagged

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Cat.No. : NEFL-23H
Product Overview : NEFL Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain (2-543 a.a) containing 551 amino acids including a 9 a.a N-terminal His tag.
Description : Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Source : E.coli
Species : Human
Tag : His
Form : Filtered White lyophilized (freeze-dried) powder.
Molecular Mass : 62.5kDa
AA Sequence : MKHHHHHHASSFSYE PYYSTSYKRRYVETP RVHISSVRSGYSTAR SAYSSYSAPVSSSLS VRRSYSSSSGSLMPS LENLDLSQVAAISND LKSIRTQEKAQLQDL NDRFASFIERVHELE QQNKVLEAELLVLRQ KHSEPSRFRALYEQE IRDLRLAAEDATNEK QALQGEREGLEETLR NLQARYEEEVLSRED AEGRLMEARKGADEA ALARAELEKRIDSLM DEISFLKKVHEEEIA ELQAQIQYAQISVEM DVTKPDLSAALKDIR AQYEKLAAKNMQNAE EWFKSRFTVLTESAA KNTDAVRAAKDEVSE SRRLLKAKTLEIEAC RGMNEALEKQLQELE DKQNADISAMQDTIN KLENELRTTKSEMAR YLKEYQDLLNVKMAL DIEIAAYRKLLEGEE TRLSFTSVGSITSGY SQSSQVFGRSAYGGL QTSSYLMSTRSFPSY YTSHVQEEQIEVEET IEAAKAEEAKDEPPS EGEAEEEEKDKEEAE EEEAAEEEEAAKEES EEAKEEEEGGEGEEG EETKEAEEEEKKVEG AGEEQAAKKKD.
Purity : Greater than 90.0% as determined by SDS-PAGE.
Stability : Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 centigrade for a limited period of time; it does not show any change after two weeks at 4 centigrade.
Storage : Store lyophilized protein at -20 centigrade.
Storage Buffer : NEFL filtered (0.4 µm) and lyophilized from 0.5 mg/mL solution in 85 mM, 15 mM Tris and Glycine, pH 8.5.
Dilutions : It is recommended to add 200 µL of deionized waterto prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely.
Shipping : Shipped at Room temp.
Gene Name : NEFL neurofilament light [ Homo sapiens (human) ]
Official Symbol : NEFL
Synonyms : NEFL; neurofilament light; NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110; neurofilament light polypeptide; light molecular weight neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; protein phosphatase 1, regulatory subunit 110
Gene ID : 4747
mRNA Refseq : NM_006158
Protein Refseq : NP_006149
MIM : 162280
UniProt ID : P07196

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