Recombinant Human NEFL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NEFL-526H
• Product Overview: NEFL MS Standard C13 and N15-labeled recombinant protein (NP_006149) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 61.5 kDa
• AA Sequence: MSSFSYEPYYSTSYKRRYVETPRVH ISSVRSGYSTARSAYSSYSAPVSSS LSVRRSYSSSSGSLMPSLENLDLSQ VAAISNDLKSIRTQEKAQLQDLNDR FASFIERVHELEQQNKVLEAELLVL RQKHSEPSRFRALYEQEIRDLRLAA EDATNEKQALQGEREGLEETLRNLQ ARYEEEVLSREDAEGRLMEARKGAD EAALARAELEKRIDSLMDEISFLKK VHEEEIAELQAQIQYAQISVEMDVT KPDLSAALKDIRAQYEKLAAKNMQN AEEWFKSRFTVLTESAAKNTDAVRA AKDEVSESRRLLKAKTLEIEACRGM NEALEKQLQELEDKQNADISAMQDT INKLENELRTTKSEMARYLKEYQDL LNVKMALDIEIAAYRKLLEGEETRL SFTSVGSITSGYSQSSQVFGRSAYG GLQTSSYLMSTRSFPSYYTSHVQEE QIEVEETIEAAKAEEAKDEPPSEGE AEEEEKDKEEAEEEEAAEEEEAAKE ESEEAKEEEEGGEGEEGEETKEAEE EEKKVEGAGEEQAAKKKDTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NEFL neurofilament light [ Homo sapiens (human) ]
• Official Symbol: NEFL
• Synonyms: NEFL; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament light polypeptide; CMT1F; CMT2E; NF68; NFL; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; light molecular weight neurofilament protein; NF-L; FLJ53642
• Gene ID: 4747
• mRNA Refseq: NM_006158
• Protein Refseq: NP_006149
• MIM: 162280
• UniProt ID: P07196