Recombinant Human NEK6, His-tagged

• Cat.No.: NEK6-559H
• Product Overview: Recombinant human NEK6 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal His tag. MW=40.1kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.
• Purity: >90 % by SDS-Page.
• Specific Activity: 70 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 ug/ml NEK6.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 1 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: NEK6 NIMA (never in mitosis gene a)-related kinase 6 [ Homo sapiens ]
• Synonyms: NEK6; NIMA (never in mitosis gene a)-related kinase 6; SID6-1512; NIMA-related kinase 6; OTTHUMP00000022097; putative serine-threonine protein kinase; EC 2.7.11.1
• Gene ID: 10783
• mRNA Refseq: NM_001145001
• Protein Refseq: NP_001138473
• MIM: 604043
• UniProt ID: P51955
• Chromosome Location: 9q33.3-q34.11
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity; protein serine/threonine kinase activity; signal transducer activity