Recombinant Human NEK7 protein(Met1-Ser302), His&GST-tagged

• Cat.No.: NEK7-761H
• Product Overview: Recombinant Human NEK7 (NP_598001.1) (Met1-Ser 302) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-302 a.a.
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.5, 0.5mM Reduced Glutathione, 0.5mM PMSF Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human NEK7/GST chimera consists of 539 amino acids and has a calculated molecular mass of 62.4 kDa. It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NEK7 NIMA (never in mitosis gene a)-related kinase 7 [ Homo sapiens ]
• Official Symbol: NEK7
• Synonyms: NEK7; NIMA (never in mitosis gene a)-related kinase 7; serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7
• Gene ID: 140609
• mRNA Refseq: NM_133494
• Protein Refseq: NP_598001
• MIM: 606848
• UniProt ID: Q8TDX7