Recombinant Human NELFB Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NELFB-4504H
• Product Overview: COBRA1 MS Standard C13 and N15-labeled recombinant protein (NP_056271) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2), either NELFC or NELFD (TH1L), and NELFE (RDBP). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 65.5 kDa
• AA Sequence: MFAGLQDLGVANGEDLKETLTNCTE PLKAIEQFQTENGVLLPSLQSALPF LDLHGTPRLEFHQSVFDELRDKLLE RVSAIASEGKAEERYKKLEDLLEKS FSLVKMPSLQPVVMCVMKHLPKVPE KKLKLVMADKELYRACAVEVKRQIW QDNQALFGDEVSPLLKQYILEKESA LFSTELSVLHNFFSPSPKTRRQGEV VQRLTRMVGKNVKLYDMVLQFLRTL FLRTRNVHYCTLRAELLMSLHDLDV GEICTVDPCHKFTWCLDACIRERFV DSKRARELQGFLDGVKKGQEQVLGD LSMILCDPFAINTLALSTVRHLQEL VGQETLPRDSPDLLLLLRLLALGQG AWDMIDSQVFKEPKMEVELITRFLP MLMSFLVDDYTFNVDQKLPAEEKAP VSYPNTLPESFTKFLQEQRMACEVG LYYVLHITKQRNKNALLRLLPGLVE TFGDLAFGDIFLHLLTGNLALLADE FALEDFCSSLFDGFFLTASPRKENV HRHALRLLIHLHPRVAPSKLEALQK ALEPTGQSGEAVKELYSQLGEKLEQ LDHRKPSPAQAAETPALELPLPSVP APAPLTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NELFB negative elongation factor complex member B [ Homo sapiens (human) ]
• Official Symbol: NELFB
• Synonyms: NELFB; COBRA1; NELF-B; RP13-122B23.3; negative elongation factor complex member B; negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B
• Gene ID: 25920
• mRNA Refseq: NM_015456
• Protein Refseq: NP_056271
• MIM: 611180
• UniProt ID: Q8WX92