Recombinant Human NEMO Protein, His-tagged


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Recombinant Human NEMO Protein, His-tagged

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Cat.No. : NEMO-371H
Product Overview : Recombinant Human NEMO Protein is produced by Bacteria expression system. This protein is fused with a His tag.
Description : 6xHis was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.
Source : Bacteria
Species : Human
Tag : His
Form : 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Molecular Mass : 18.4 kDa
Purity : > 95 % by SDS-PAGE
Notes : NEMO (UBAN) may also bind long polyubiquitin chains linked via lysine 63.
Storage : Store at -80 centigrade; avoid multiple freeze-thaw cycles.
Synonyms : IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma

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