Recombinant Human NEMO Protein, His-tagged

• Cat.No.: NEMO-371H
• Product Overview: Recombinant Human NEMO Protein is produced by Bacteria expression system. This protein is fused with a His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: 6xHis was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.
• Form: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
• Molecular Mass: 18.4 kDa
• Purity: > 95 % by SDS-PAGE
• Notes: NEMO (UBAN) may also bind long polyubiquitin chains linked via lysine 63.
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.

Gene Information

• Synonyms: IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma