| Cat.No. : |
NEMO-371H |
| Product Overview : |
Recombinant Human NEMO Protein is produced by Bacteria expression system. This protein is fused with a His tag. |
| Description : |
6xHis was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole. |
| Source : |
Bacteria |
| Species : |
Human |
| Tag : |
His |
| Form : |
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Molecular Mass : |
18.4 kDa |
| Purity : |
> 95 % by SDS-PAGE |
| Notes : |
NEMO (UBAN) may also bind long polyubiquitin chains linked via lysine 63. |
| Storage : |
Store at -80 centigrade; avoid multiple freeze-thaw cycles. |
| Synonyms : |
IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma |
