Recombinant Human Neutrophil Cytosolic Factor 1, His-tagged

• Cat.No.: NCF1-1721H
• Product Overview: Recombinant humanNCF1 protein, fused to His-tag at C-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Neutrophil cytosolicfactor 1, also known as NCF1, is a cytosolic subunit of neutrophil NADPHoxidase. NCF1, along with NCF2 and a membrane bound cytochrome b558, isrequired for activation of the latent NADPH oxidase necessary for superoxideproduction. Defects in NCF1, often resulting from recombination between NCF1and a nearby homologous pseudogene, cause chronic granulomatous disease.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquidin 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT, 20% glycerol and 0.1 MNaCl.
• Purity: > 90% pure bySDS-PAGE
• Sequence: 1-390 amino acids:MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENRIIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYLMPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFLEPLD
• Molecular Mass: 45.7 kDa (398 aa)confirmed by MALDI-TOF
• Applications: SDS-PAGE
• Storage: Store at 4 deg C forshort term storage (1/2 weeks). Aliquot and store at -20 deg C or -70 deg Cfor long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: NCF1

Gene Information

• Gene Name: NCF1neutrophil cytosolic factor 1 [ Homo sapiens ]
• Synonyms: NCF1; neutrophil cytosolic factor 1;NCF1A; NOXO2; p47phox; FLJ79451; SH3PXD1A; NCF-1; NCF-47K; p47-phox; nox organizer 2; OTTHUMP00000195569; nox-organizing protein 2; NADPH oxidase organizer 2; 47 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 1; SH3 and PX domain-containing protein 1A; 47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
• Gene ID: 653361
• mRNA Refseq: NM_000265
• Protein Refseq: NP_000256
• MIM: 608512
• UniProt ID: P14598
• Chromosome Location: 7q11.23
• Pathway: Chemokine signalingpathway; Fc gamma R-mediated phagocytosis; Leishmaniasis; Leukocyte transendothelial migration; Osteoclastdifferentiation; Phagosome; RAC1 signaling pathway
• Function: superoxide-generatingNADPH oxidase activity; protein binding; phosphatidylinositol binding;electron carrier activity; SH3 domain binding; GTPase activity; GTP binding