Recombinant Human Neutrophil Cytosolic Factor 4, 40kDa, T7-tagged

• Cat.No.: NCF4-2953H
• Product Overview: Recombinant Human NCF4 was produced inE.coliwith a T7 tag at N-terminus. MW = 39032 Da (1-348 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Protein Length: 1-348 a.a.
• Description: Neutrophil cytosol factor 4 is a protein that in humans is encoded by the NCF4 gene. The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT.
• Purity: 95%.
• Applications: MS. SDS.

Gene Information

• Gene Name: NCF4 neutrophil cytosolic factor 4, 40kDa [ Homo sapiens ]
• Synonyms: NCF4; neutrophil cytosolic factor 4, 40kDa; NCF; MGC3810; P40PHOX; SH3PXD4; NCF-4; p40-phox; neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; neutrophil cytosolic factor 4 (40kD)
• Gene ID: 4689
• mRNA Refseq: NM_000631
• Protein Refseq: NP_000622
• MIM: 601488
• UniProt ID: Q15080
• Chromosome Location: 22q13.1
• Pathway: Leishmaniasis; Leukocyte transendothelial migration; Phagosome
• Function: phosphoinositide binding; protein binding; protein dimerization activity