Recombinant Human NHE1 protein, His-tagged

• Cat.No.: NHE1-3792H
• Product Overview: Recombinant Human NHE1 protein(1-108 aa), fused to His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-108 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AA Sequence: MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLW
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SLC9A1 solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1 [ Homo sapiens (human) ]
• Official Symbol: NHE1
• Synonyms: SLC9A1; APNH; NHE1; NHE-1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1; sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)
• Gene ID: 6548
• mRNA Refseq: NM_003047
• Protein Refseq: NP_003038
• MIM: 107310
• UniProt ID: P19634