Recombinant Human Nicotinamide N-methyltransferase, His-tagged

• Cat.No.: NNMT-143H
• Product Overview: Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 31.7 kDa (284aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDYLEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQLL SAC E SFKE IVVTD YSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCVLSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNEGLFSLVARKL SRPL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Noncompetitive Inhibition of Indolethylamine N-Methyltransferase by N, N Dimethyltryptamine (DMT) and N, N-Dimethylaminopropyltryptamine (2014)

Gene Information

• Gene Name: NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
• Synonyms: NNMT; nicotinamide N-methyltransferase; EC 2.1.1.1.
• Gene ID: 4837
• mRNA Refseq: NM_006169
• Protein Refseq: NP_006160
• MIM: 600008
• UniProt ID: P40261
• Chromosome Location: 11q23.1
• Pathway: Nicotinate and nicotinamide metabolism ; Biological oxidations
• Function: methyltransferase activity; nicotinamide N-methyltransferase activity; transferase activity

Citation

Noncompetitive Inhibition of Indolethylamine- N -methyltransferase by N , N -Dimethyltryptamine and N , N -Dimethylaminopropyltryptamine

Journal: Biochemistry    PubMed ID: 24730580    Data: 2015/4/14

Authors: Uyen B. Chu, Sevahn K. Vorperian, Arnold E. Ruoho

Article Snippet:Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).Histidine-tagged human indolethylamine- N -methyltransferase was obtained from the Structural Genomics Consortium (University of Toronto, Toronto, ON).. Recombinant human phenylethanolamine- N -methyltransferase (hPNMT) and nicotinamide- N -methyltransferase (hNNMT) were purchased from Creative BioMart (Shirley, NY).. All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).All reagents for the hPNMT assay were prepared in 50 mM Tris-HCl buffer (pH 8.5) unless stated otherwise, excluding PDAT, which was dissolved in dimethyl sulfoxide (DMSO).