Recombinant Human Nicotinamide Riboside Kinase 1 Isoform 1, His-tagged

• Cat.No.: NRK1-6793H
• Product Overview: Recombinant humanNRK1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NRK1 belongs to theuridine kinase family. NAD+ is essential for life in all organisms, both as acoenzyme for oxidoreductases and as a source of ADPribosyl groups used invarious reactions, including those that retard aging in experimental systems.Nicotinic acid and nicotinamide were defined as the vitamin precursors ofNAD+. This enzyme catalyzes the phosphorylation of nicotinamide riboside (NR)and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN)and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates theantitumor drugs tiazofurin and 3-deazaguanosine
• Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl
• Molecular Weight: 25.6kDa (222aa),confirmed by MALDI-TOF
• Purity: > 90% by SDS -PAGE
• Concentration: 0.5mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMKTFIIG ISGVTNSGKT TLAKNLQKHL PNCSVISQDD FFKPESEIET DKNGFLQYDVLEALNMEKMM SAISCWMESA RHSVVSTDQE SAEEIPILII EGFLLFNYKP LDTIWNRSYF LTIPYEECKRRRSTRVYQPP DSPGYFDGHV WPMYLKYRQE MQDITWEVVY LDGTKSEEDL FLQVYEDLIQ ELAKQKCLQVTA
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: C9orf95 chromosome 9 openreading frame 95 [Homo sapiens]
• Official symbol: C9orf95
• Synonyms: chromosome9 open reading frame 95; Ribosylnicotinic acid kinase 1; FLJ20559; NRK 1;NRK1; RNK 1; bA235O14.2; OTTHUMP00000021494; Nicotinic acid riboside kinase1; nicotinamide riboside kinase 1; NmR-K 1; EC 2.7.1.22; Ribosylnicotinamidekinase 1; EC 2.7.1.n4; RP11-235O14.2; OTTHUMP00000021493; OTTHUMP00000021495
• Gene ID: 54981
• mRNA Refseq: NM_017881
• Protein Refseq: NP_060351
• MIM: 608704
• Uniprot ID: Q9NWW6
• Chromosome Location: 9q21.13
• Pathway: Nicotinate and nicotinamide metabolism, organism-specificbiosystem; Nicotinate and nicotinamide metabolism, conserved biosystem
• Function: ATPbinding; metal ion binding; nucleotide binding; ribosylnicotinamide kinaseactivity; transferase activity