Recombinant Human Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein

• Cat.No.: NOS1AP-636H
• Product Overview: Recombinant Human Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein is producedinSf9 cells.MW=160kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins.
• Formulation: Liquid. In 50mM triethanolamine, pH 7.5, 0.5mM EDTA, 7mM GSH, 0.2% CHAPSO, 1µm E64, 1µM leupeptin, 1µM pepstatin, 1mg/ml trypsin inhibitor and 10% glycerol.
• Specific Activity: ≥1nmol/100μl/min (U/100μl). Amount assayed 1µl. Optimal conditions: 37°C, pH 7.0, 1mM L-arginine, 1mM NADPH, 10µM (6R)-tetrahydrobiopterin, 5µM FAD, 5µM FMN, 50nM calmodulin, 1mM CaCl2 and 7mM GSH.
• Storage: -80°C. nNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle.

Gene Information

• Gene Name: NOS1AP nitric oxide synthase 1 (neuronal) adaptor protein [ Homo sapiens ]
• Synonyms: NOS1AP; nitric oxide synthase 1 (neuronal) adaptor protein; C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON); C-terminal PDZ ligand of neuronal nitric oxide synthase protein; carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain; nitric oxide synthase 1 adaptor protein; CAPON; MGC138500; 6330408P19Rik
• Gene ID: 9722
• mRNA Refseq: NM_001126060
• Protein Refseq: NP_001119532
• MIM: 605551
• UniProt ID: O75052
• Chromosome Location: 1q23.3
• Function: PDZ domain binding; nitric-oxide synthase binding; protein binding