Recombinant Human NLRP1 Protein (L1379-K1462), His tagged

• Cat.No.: NLRP1-0068H
• Product Overview: Recombinant Human 6His-TEV-NLRP1(L1379-K1462) Protein was expressed in E. coli.

Specification

• Description: Acts as the sensor component of the NLRP1 inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as cleavage by human rhinoviruses 14 and 16 (HRV-14 and HRV-16), double-stranded RNA or Val-boroPro inhibitor, and mediates the formation of the inflammasome polymeric complex composed of NLRP1, CASP1 and PYCARD/ASC. In response to pathogen-associated signals, the N-terminal part of NLRP1 is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (NACHT, LRR and PYD domains-containing protein 1, C-terminus), which polymerizes and associates with PYCARD/ASC to initiate the formation of the inflammasome complex: the NLRP1 inflammasome recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis. Activation of NLRP1 inflammasome is also required for HMGB1 secretion; the active cytokines and HMGB1 stimulate inflammatory responses. Binds ATP and shows ATPase activity. Plays an important role in antiviral immunity and inflammation in the human airway epithelium. Specifically recognizes a number of pathogen-associated signals: upon infection by human rhinoviruses 14 and 16 (HRV-14 and HRV-16), NLRP1 is cleaved and activated which triggers NLRP1-dependent inflammasome activation and IL18 secretion. Positive-strand RNA viruses such as. Semliki forest virus and long dsRNA activate the NLRP1 inflammasome, triggering IL1B release in a NLRP1-dependent fashion. Acts as a direct sensor for long dsRNA and thus RNA virus infection. May also be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein Length: L1379-K1462
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: NLRP1 NLR family, pyrin domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: NLRP1
• Synonyms: NLRP1; NLR family, pyrin domain containing 1; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1, NALP1; NACHT, LRR and PYD domains-containing protein 1; CARD7; CLR17.1; DEFCAP; DKFZp586O1822; KIAA0926; NAC; nucleotide binding oligomerization domain; leucine rich repeat and pyrin domain containing 1; caspase recruitment domain protein 7; NACHT, LRR and PYD containing protein 1; NACHT, leucine rich repeat and PYD containing 1; caspase recruitment domain-containing protein 7; nucleotide-binding domain and caspase recruitment domain; death effector filament-forming Ced-4-like apoptosis protein; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1; NALP1; SLEV1; PP1044; VAMAS1; DEFCAP-L/S
• Gene ID: 22861
• mRNA Refseq: NM_001033053
• Protein Refseq: NP_001028225
• MIM: 606636
• UniProt ID: Q9C000