Recombinant Human NMT1 Protein, GST-tagged

• Cat.No.: NMT1-5945H
• Product Overview: Human NMT1 full-length ORF ( NP_066565.1, 1 a.a. - 496 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 83.2 kDa
• AA Sequence: MADESETAVKPPAPPLPQMMEGNGN GHEHCSDCENEEDNSYNRGGLSPAN DTGAKKKKKKQKKKKEKGSETDSAQ DQPVKMNSLPAERIQEIQKAIELFS VGQGPAKTMEEASKRSYQFWDTQPV PKLGEVVNTHGPVEPDKDNIRQEPY TLPQGFTWDALDLGDRGVLKELYTL LNENYVEDDDNMFRFDYSPEFLLWA LRPPGWLPQWHCGVRVVSSRKLVGF ISAIPANIHIYDTEKKMVEINFLCV HKKLRSKRVAPVLIREITRRVHLEG IFQAVYTAGVVLPKPVGTCRYWHRS LNPRKLIEVKFSHLSRNMTMQRTMK LYRLPETPKTAGLRPMETKDIPVVH QLLTRYLKQFHLTPVMSQEEVEHWF YPQENIIDTFVVENANGEVTDFLSF YTLPSTIMNHPTHKSLKAAYSFYNV HTQTPLLDLMSDALVLAKMKGFDVF NALDLMENKTFLEKLKFGIGDGNLQ YYLYNWKCPSMGAEKVGLVLQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: NMT1 N-myristoyltransferase 1 [ Homo sapiens ]
• Official Symbol: NMT1
• Synonyms: NMT1; N-myristoyltransferase 1; glycylpeptide N-tetradecanoyltransferase 1; alternative; short form NMT S; long form; NMT L; myristoyl CoA:protein N myristoyltransferase; NMT; NMT 1; long form, NMT-L; alternative, short form NMT-S; type I N-myristoyltransferase; peptide N-myristoyltransferase 1; myristoyl-CoA:protein N-myristoyltransferase 1
• Gene ID: 4836
• mRNA Refseq: NM_021079
• Protein Refseq: NP_066565
• MIM: 160993
• UniProt ID: P30419