Recombinant Human NOB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NOB1-1357H
• Product Overview: NOB1 MS Standard C13 and N15-labeled recombinant protein (NP_054781) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.7 kDa
• AA Sequence: MAPVEHVVADAGAFLRHAALQDIGK NIYTIREVVTEIRDKATRRRLAVLP YELRFKEPLPEYVRLVTEFSKKTGD YPSLSATDIQVLALTYQLEAEFVGV SHLKQEPQKVKVSSSIQHPETPLHI SGFHLPYKPKPPQETEKGHSACEPE NLEFSSFMFWRNPLPNIDHELQELL IDRGEDVPSEEEEEEENGFEDRKDD SDDDGGGWITPSNIKQIQQELEQCD VPEDVRVGCLTTDFAMQNVLLQMGL HVLAVNGMLIREARSYILRCHGCFK TTSDMSRVFCSHCGNKTLKKVSVTV SDDGTLHMHFSRNPKVLNPRGLRYS LPTPKGGKYAINPHLTEDQRFPQLR LSQKARQKTNVFAPDYIAGVSPFVE NDISSRSATLQVRDSTLGAGRRRLN PNASRKKFVKKRTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NOB1 NIN1 (RPN12) binding protein 1 homolog [ Homo sapiens (human) ]
• Official Symbol: NOB1
• Synonyms: NOB1; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); PSMD8 binding protein 1, PSMD8BP1; RNA-binding protein NOB1; ART 4; MST158; nin one binding protein; NOB1P; protein ART-4; PSMD8 binding protein 1; phosphorylation regulatory protein HP-10; adenocarcinoma antigen recognized by T lymphocytes 4; ART-4; MSTP158; PSMD8BP1
• Gene ID: 28987
• mRNA Refseq: NM_014062
• Protein Refseq: NP_054781
• MIM: 613586
• UniProt ID: Q9ULX3