Recombinant Human NPEPPS protein, His-tagged

• Cat.No.: NPEPPS-1158H
• Product Overview: Recombinant Human NPEPPS aa. (Gly584~Gln793 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly584~Gln793
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.0.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose and 0.01% sarcosyl.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NPEPPS aminopeptidase puromycin sensitive [ Homo sapiens (human) ]
• Official Symbol: NPEPPS
• Synonyms: NPEPPS; aminopeptidase puromycin sensitive; PSA; AAP-S; MP100; puromycin-sensitive aminopeptidase; cytosol alanyl aminopeptidase; metalloproteinase MP100
• Gene ID: 9520
• mRNA Refseq: NM_001330257.1
• Protein Refseq: NP_001317186.1
• UniProt ID: P55786