Active Recombinant Human NRP2 protein, His-tagged

• Cat.No.: NRP2-818H
• Product Overview: Active Recombinant Human NRP2 protein(Met 1-Tyr 855)(NP_003863.2) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-855 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Neuropilin-2 at 2.5 μg/ml (100 μl/well) can bind recombinant human VEGF165 and EC50 value is 0.57 nM.
• Molecular Mass: The recombinant human NRP2 consists of 844 amino acids and has a calculated molecular mass of 95 kDa. The apparent molecular mass of rhNRP2 is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP2 neuropilin 2 [ Homo sapiens ]
• Official Symbol: NRP2
• Synonyms: NRP2; neuropilin 2; neuropilin-2; VEGF165R2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2; NP2; NPN2; PRO2714; MGC126574
• Gene ID: 8828
• mRNA Refseq: NM_003872
• Protein Refseq: NP_003863
• MIM: 602070
• UniProt ID: O60462