Active Recombinant Human NRXN3 Protein, His-tagged

• Cat.No.: NRXN3-015H
• Product Overview: Active Recombinant Human NRXN3 Protein(NP_620426.2)(Met 1-Thr 357) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-357 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to NRXN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37 °C.
• Molecular Mass: The recombinant human NRXN3 consists of 333 amino acids and predictes a molecular mass of 36 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhNRXN3 is approximately 50-60 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRXN3 neurexin 3 [ Homo sapiens ]
• Official Symbol: NRXN3
• Synonyms: NRXN3; neurexin 3; C14orf60, chromosome 14 open reading frame 60; neurexin-3-beta; KIAA0743; neurexin III; neurexin III-beta; neurexin III-alpha; C14orf60; MGC176711
• Gene ID: 9369
• mRNA Refseq: NM_001105250
• Protein Refseq: NP_001098720
• MIM: 600567
• UniProt ID: Q9HDB5