Recombinant Human NTRK1, Fc-tagged

• Cat.No.: NTRK1-495H
• Product Overview: Recombinant Human NTRK1 encoding the signal peptide from human CD33 joined with the extracellular domain of human Trk A (Ala 33 - Glu 407) was fused to the Fc region of human IgG1 via a polypeptide linker and expressed inNSO cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 33-407 a.a.
• Description: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer.
• Molecular Weight: The predicted molecular weight of Recombinant Human is Mr 67.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 115-125 kDa.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Biological Activity: The biological activity of Human TrkA was determined by its ability to inhibit NGF-induced proliferation of TF1 cells. The ED50 for this effect is typically 0.8 - 4 μg/mL in the presence of 10 ng/mL of rhNGF.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens ]
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; MTC; TRK; TRK1; TRKA; p140-TrkA; DKFZp781I14186; Oncogene TRK; tyrosine kinase receptor A; high affinity nerve growth factor receptor; EC 2.7.10.1; OTTHUMP00000038736; Trk-A; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• Protein Refseq: NP_001007793
• MIM: 191315
• UniProt ID: P04629
• Chromosome Location: 1q21-q22
• Pathway: Apoptosis; Endocytosis; MAPK signaling pathway; Neurotrophin signaling pathway; Pathways in cancer; Thyroid cancer; Signalling by NGF
• Function: ATP binding; neurotrophin receptor activity; nucleotide binding; protein binding; transferase activity; transmembrane receptor protein tyrosine kinase activity