Recombinant Human NTRK2 protein, His-tagged

• Cat.No.: NTRK2-157H
• Product Overview: Recombinant Human NTRK2 protein(AAH31835)(Cys32-His430), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Cys32-His430
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human/Murine/Rat BDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.24μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NTRK2 neurotrophic tyrosine kinase, receptor, type 2 [ Homo sapiens ]
• Official Symbol: NTRK2
• Synonyms: NTRK2; neurotrophic tyrosine kinase, receptor, type 2; BDNF/NT-3 growth factors receptor; TRKB; trkB tyrosine kinase; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B; trk-B; GP145-TrkB
• Gene ID: 4915
• mRNA Refseq: NM_001007097
• Protein Refseq: NP_001007098
• MIM: 600456
• UniProt ID: Q16620