Recombinant Human nucleic acid binding protein 1, His-tagged

• Cat.No.: NABP1-270H
• Product Overview: NABP1, 1-204aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-204 a.a.
• Description: Nucleic acid binding protein 1, also known as NABP1, is component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, the protein acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. This protein is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. Recombinant human NABP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Mass: 24.8 kDa (227aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNRVNDP LIFIRDIKPG LKNLNVVFIV LEIGRVTKTK DGHEVRSCKV ADKTGSITIS VWDEIGGLIQ PGDIIRLTRG YASMWKGCLT LYTGRGGELQ KIGEFCMVYS EVPNFSEPNP DYRGQQNKGA QSEQKNNSMN SNMGTGTFGP VGNGVHTGPE SREHQFSHAG RSNGRGLINP QLQGTASNQT VMTTISNGRD PRRAFKR
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NABP1 nucleic acid binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: NABP1
• Synonyms: NABP1; SSB2; OBFC2A; SOSS-B2; nucleic acid binding protein 1; SOSS complex subunit B2; sensor of ssDNA subunit B2; nucleic acid-binding protein 1; single-stranded DNA-binding protein 2; sensor of single-strand DNA complex subunit B2; oligonucleotide/oligosaccharide-binding fold containing 2A; oligonucleotide/oligosaccharide-binding fold-containing protein 2A
• Gene ID: 64859
• mRNA Refseq: NM_001031716
• Protein Refseq: NP_001026886
• MIM: 612103
• UniProt ID: Q96AH0
• Chromosome Location: 2q32.3
• Function: RNA binding; single-stranded DNA binding