Recombinant Human Nucleosome, H3K9me2 dNuc, Biotinylated
Cat.No. : | NUC-018 |
Product Overview : | Mononucleosomes assembled from recombinant human histones expressed in E. coli. |
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Description : | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains dimethyl-lysine at position 9. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. * H3K9me2 has a Cys to Ala substitution at position 110. |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | Nucleosome, Recombinant Human, H3K9me2 (27.3 µg protein weight, 50 µg total weight) in 50 µl of 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 4.99 μmolar. |
Molecular Mass : | 200,493 Da |
Applications : | Nucleosome, Recombinant Human, H3K9me2 dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. |
Storage : | Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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