Recombinant Human NUMB Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: NUMB-393H
• Product Overview: NUMB MS Standard C13 and N15-labeled recombinant protein (NP_001005744) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 65.7 kDa
• AA Sequence: MNKLRQSFRRKKDVYVPEASRPHQW QTDEEGVRTGKCSFPVKYLGHVEVD ESRGMHICEDAVKRLKAERKFFKGF FGKTGKKAVKAVLWVSADGLRVVDE KTKDLIVDQTIEKVSFCAPDRNFDR AFSYICRDGTTRRWICHCFMAVKDT GERLSHAVGCAFAACLERKQKREKE CGVTATFDASRTTFTREGSFRVTTA TEQAEREEIMKQMQDAKKAETDKIV VGSSVAPGNTAPSPSSPTSPTSDAT TSLEMNNPHAIPRRHAPIEQLARQG SFRGFPALSQKMSPFKRQLSLRINE LPSTMQRKTDFPIKNAVPEVEGEAE SISSLCSQITNAFSTPEDPFSSAPM TKPVTVVAPQSPTFQGTEWGQSSGA ASPGLFQAGHRRTPSEADRWLEEVS KSVRAQQPQASAAPLQPVLQPPPPT AISQPASPFQGNAFLTSQPVPVGVV PALQPAFVPAQSYPVANGMPYPAPN VPVVGITPSQMVANVFGTAGHPQAA HPHQSPSLVRQQTFPHYEASSATTS PFFKPPAQHLNGSAAFNGVDDGRLA SADRHTEVPTGTCPVDPFEAQWAAL ENKSKQRTNPSPTNPFSSDLQKTFE IELTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NUMB numb homolog (Drosophila) [ Homo sapiens (human) ]
• Official Symbol: NUMB
• Synonyms: NUMB; numb homolog (Drosophila); C14orf41, chromosome 14 open reading frame 41, numb (Drosophila) homolog; protein numb homolog; h-Numb; S171; C14orf41; c14_5527; FLJ31314
• Gene ID: 8650
• mRNA Refseq: NM_001005744
• Protein Refseq: NP_001005744
• MIM: 603728
• UniProt ID: P49757