Recombinant Human NUP35 protein, His & T7-tagged

• Cat.No.: NUP35-1486H
• Product Overview: Recombinant Human NUP35 aa. (Thr132~Lys310 (Accession # Q8NFH5)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.4kDa.
• Protein length: Thr132~Lys310 (Accession # Q8NFH5)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NUP35 nucleoporin 35 [ Homo sapiens (human) ]
• Official Symbol: NUP35
• Synonyms: MP44; NP44; MP-44; NUP53; mitotic phosphoprotein 44; nuclear pore complex protein Nup53; nucleoporin 35kDa; nucleoporin NUP53
• Gene ID: 129401
• mRNA Refseq: NM_001287584.1
• Protein Refseq: NP_001274513.1
• UniProt ID: Q8NFH5