| Description : |
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
hFC |
| Form : |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Molecular Mass : |
116 kDa |
| Protein length : |
Partial |
| AA Sequence : |
MFNKSFGTPFGGGTGGFGTTSTFGQ NTGFGTTSGGAFGTSAFGSSNNTGG LFGNSQTKPGGLFGTSSFSQPATST STGFGFGTSTGTANTLFGTASTGTS LFSSQNNAFAQNKPTGFGNFGTSTS SGGLFGTTNTTSNPFGSTSGSLFGP SSFTAAPTGTTIKFNPPTGTDTMVK AGVSTNISTKHQCITAMKEYESKSL EELRLEDYQANRKGPQNQVGAGTTT GLFGSSPATSSATGLFSSSTTNSGF AYGQNKTAFGTSTTGFGTNPGGLFG QQNQQTTSLFSKPFGQATTTQNTGF SFGNTSTIGQPSTNTMGLFGVTQAS QPGGLFGTATNTSTGTAFGTGTGLF GQTNTGFGAVGSTLFGNNKLTTFGS STTSAPSFGTTSGGLFGNKPTLTLG TNTNTSNFGFGTNTSGNSIFGSKPA PGTLGTGLGAGFGTALGAGQASLFG NNQPKIGGPLGTGAFGAPGFNTTTA TLGFGAPQAPVALTDPNASAAQQAV LQQHINSLTYSPFGDSPLFRNPMSD PKKKEERLKPTNPAAQKALTTPTHY KLTPRPATRVRPKALQTTGTAKSHL FDGLDDDEPSLANGAFMPKKSIKKL VLKNLNNSNLFSPVNRDSENLASPS EYPENGERFSFLSKPVDENHQQDGD EDSLVSHFYTNPIAKPIPQTPESAG NKHSNSNSVDDTIVALNMRAALRNG LEGSSEETSFHDESLQDDREEIENN SYHMHPAGIILTKVGYYTIPSMDDL AKITNEKGECIVSDFTIGRKGYGSI YFEGDVNLTNLNLDDIVHIRRKEVV VYLDDNQKPPVGEGLNRKAEVTLDG VWPTDKTSRCLIKSPDRLADINYEG RLEAVSRKQGAQFKEYRPETGSWVF KVSHF |
| Purity : |
Greater than 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Storage : |
Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
