Description : |
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
51.1 kDa |
AA Sequence : |
MNNFGNEEFDCHFLDEGFTAKDILD QKINEVSSSDDKDAFYVADLGDILK KHLRWLKALPRVTPFYAVKCNDSKA IVKTLAATGTGFDCASKTEIQLVQS LGVPPERIIYANPCKQVSQIKYAAN NGVQMMTFDSEVELMKVARAHPKAK LVLRIATDDSKAVCRLSVKFGATLR TSRLLLERAKELNIDVVGVSFHVGS GCTDPETFVQAISDARCVFDMGAEV GFSMYLLDIGGGFPGSEDVKLKFEE ITGVINPALDKYFPSDSGVRIIAEP GRYYVASAFTLAVNIIAKKIVLKEQ TGSDDEDESSEQTFMYYVNDGVYGS FNCILYDHAHVKPLLQKRPKPDEKY YSSSIWGPTCDGLDRIVERCDLPEM HVGDWMLFENMGAYTVAAASTFNGF QRPTIYYVMSGPAWQLMQQFQNPDF PPEVEEQDASTLPVSCAWESGMKRH RAACASASINVTRTRPLEQKLISEE DLAANDILDYKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |