| Description : |
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
115.9 kDa |
| AA Sequence : |
MFHLRTCAAKLRPLTASQTVKTFSQ NRPAAARTFQQIRCYSAPVAAEPFL SGTSSNYVEEMYCAWLENPKSVHKS WDIFFRNTNAGAPPGTAYQSPLPLS RGSLAAVAHAQSLVEAQPNVDKLVE DHLAVQSLIRAYQIRGHHVAQLDPL GILDADLDSSVPADIISSTDKLGFY GLDESDLDKVFHLPTTTFIGGQESA LPLREIIRRLEMAYCQHIGVEFMFI NDLEQCQWIRQKFETPGIMQFTNEE KRTLLARLVRSTRFEEFLQRKWSSE KRFGLEGCEVLIPALKTIIDKSSEN GVDYVIMGMPHRGRLNVLANVIRKE LEQIFCQFDSKLEAADEGSGDVKYH LGMYHRRINRVTDRNITLSLVANPS HLEAADPVVMGKTKAEQFYCGDTEG KKVMSILLHGDAAFAGQGIVYETFH LSDLPSYTTHGTVHVVVNNQIGFTT DPRMARSSPYPTDVARVVNAPIFHV NSDDPEAVMYVCKVAAEWRSTFHKD VVVDLVCYRRNGHNEMDEPMFTQPL MYKQIRKQKPVLQKYAELLVSQGVV NQPEYEEEISKYDKICEEAFARSKD EKILHIKHWLDSPWPGFFTLDGQPR SMSCPSTGLTEDILTHIGNVASSVP VENFTIHGGLSRILKTRGEMVKNRT VDWALAEYMAFGSLLKEGIHIRLSG QDVERGTFSHRHHVLHDQNVDKRTC IPMNHLWPNQAPYTVCNSSLSEYGV LGFELGFAMASPNALVLWEAQFGDF HNTAQCIIDQFICPGQAKWVRQNGI VLLLPHGMEGMGPEHSSARPERFLQ MCNDDPDVLPDLKEANFDINQLYDC NWVVVNCSTPGNFFHVLRRQILLPF RKPLIIFTPKSLLRHPEARSSFDEM LPGTHFQRVIPEDGPAAQNPENVKR LLFCTGKVYYDLTRERKARDMVGQV AITRIEQLSPFPFDLLLKEVQKYPN AELAWCQEEHKNQGYYDYVKPRLRT TISRAKPVWYAGRDPAAAPATGNKK THLTELQRLLDTAFDLDVFKNFSTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
