Recombinant Human OTUB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: OTUB1-1695H
• Product Overview: OTUB1 MS Standard C13 and N15-labeled recombinant protein (NP_060140) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 31.3 kDa
• AA Sequence: MAAEEPQQQKQEPLGSDSEGVNCLA YDEAIMAQQDRIQQEIAVQNPLVSE RLELSVLYKEYAEDDNIYQQKIKDL HKKYSYIRKTRPDGNCFYRAFGFSH LEALLDDSKELQRFKAVSAKSKEDL VSQGFTEFTIEDFHNTFMDLIEQVE KQTSVADLLASFNDQSTSDYLVVYL RLLTSGYLQRESKFFEHFIEGGRTV KEFCQQEVEPMCKESDHIHIIALAQ ALSVSIQVEYMDRGEGGTTNPHIFP EGSEPKVYLLYRPGHYDILYKTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 [ Homo sapiens (human) ]
• Official Symbol: OTUB1
• Synonyms: OTUB1; OTU domain, ubiquitin aldehyde binding 1; ubiquitin thioesterase OTUB1; FLJ20113; FLJ40710; otubain-1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTB1; OTU1; HSPC263; MGC4584; MGC111158
• Gene ID: 55611
• mRNA Refseq: NM_017670
• Protein Refseq: NP_060140
• MIM: 608337
• UniProt ID: Q96FW1