Recombinant Human P4HB, His-tagged

• Cat.No.: P4HB-30545TH
• Product Overview: Recombinant Human P4HB fused at the N terminal to a His-tag, predicted Mwt: 58 kDa.

Specification

• Description: This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
• Conjugation: HIS
• Molecular Weight: 58.000kDa
• Source: E. coli
• Biological activity: >200U/mg
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 7.50Constituents:0.61% Tris, 0.88% Sodium chloride, 5% Glycerol, 0.02% DTT, 0.03% EDTA
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein disulfide isomerase family.Contains 2 thioredoxin domains.

Gene Information

• Gene Name: P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens ]
• Official Symbol: P4HB
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; ERBA2L, PO4DB, procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide , procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide (prote
• Gene ID: 5034
• mRNA Refseq: NM_000918
• Protein Refseq: NP_000909
• MIM: 176790
• Uniprot ID: P07237
• Chromosome Location: 17q25
• Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem
• Function: electron carrier activity; isomerase activity; procollagen-proline 4-dioxygenase activity; protein binding; protein disulfide isomerase activity