Recombinant Human PABPC1, His-tagged

• Cat.No.: PABPC1-30620TH
• Product Overview: Recombinant fragment, corresponding to amino acids 96-636 of Human PABP with N terminal His tag; Predicted MWt 61 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 96-636 a.a.
• Description: This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3 poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
• Conjugation: HIS
• Form: Lyophilised:Reconstitute with 149 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: SGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDEN GSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRF KSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLF GKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVD EMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRIT RYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMM EGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYV ALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPA PPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGAR PHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQR VANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRN PQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQE QKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPT V

Gene Information

• Gene Name: PABPC1 poly(A) binding protein, cytoplasmic 1 [ Homo sapiens ]
• Official Symbol: PABPC1
• Synonyms: PABPC1; poly(A) binding protein, cytoplasmic 1; PAB1, PABPC2, poly(A) binding protein, cytoplasmic 2; polyadenylate-binding protein 1; PABP1; PABPL1
• Gene ID: 26986
• mRNA Refseq: NM_002568
• Protein Refseq: NP_002559
• MIM: 604679
• Uniprot ID: P11940
• Chromosome Location: 8q22.2-q23
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Destabilization of mRNA by AUF1 (hnRNP D0), organism-specific biosystem
• Function: RNA binding; nucleotide binding; poly(A) RNA binding; poly(A) RNA binding; protein C-terminus binding