Recombinant Mouse Pabpc1 Protein, Myc/DDK-tagged

• Cat.No.: Pabpc1-4644M
• Product Overview: Purified recombinant protein of mouse full-length poly(A) binding protein, cytoplasmic 1 (Pabpc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.
• Molecular Mass: 71.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pabpc1 poly(A) binding protein, cytoplasmic 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pabpc1
• Synonyms: PABPC1; poly(A) binding protein, cytoplasmic 1; polyadenylate-binding protein 1; PABP-1; poly A binding protein 1; poly(A)-binding protein 1; PABP; ePAB; Pabp1; PabpI; Pabpl1
• Gene ID: 18458
• mRNA Refseq: NM_008774
• Protein Refseq: NP_032800
• UniProt ID: P29341