Recombinant Human PARP protein(Met1-Trp1014), His-tagged

• Cat.No.: PARP1-650H
• Product Overview: Recombinant Human PARP1 (NP_001609.2) (Met 1-Trp 1014) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: Met1-Trp1014
• Form: Supplied as sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP, 2 mM EDTA, pH 7. 5.
• Molecular Mass: The recombinant human PARP1 consists of 1024 amino acids and predicts a molecular mass of 114.5 kDa. The apparent molecular mass of rhPARP1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Discovery of novel compounds as potent activators of Sirt3 (2022)