Recombinant Human PCBD1, His-tagged

• Cat.No.: PCBD1-2809H
• Product Overview: Recombinant human PCBD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 14.1 kDa (124aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Pterin-4-alpha-carbinolamine dehydratase precursor, also known as PCBD1, is a component of the phenylalanine hydroxylase (PAH) system and participates in tetrahydrobiopterin biosynthesis. A deficiency of this enzyme leads to hyperphenylalaninemia. The enzyme regulates the homodimerization of the transcription factor hepatocyte nuclear factor 1 (HNF1).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PCBD1 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha [ Homo sapiens ]
• Synonyms: PCBD1; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; PCD; PHS; DCOH; PCBD; pterin-4-alpha-carbinolamine dehydratase; dimerizing cofactor for HNF1; dimerization cofactor of HNF1; 4-alpha-hydroxy-tetrahydropterin dehydratase; phenylalanine hydroxylase-stimulating protein; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); EC 4.2.1.96; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); Pterin-4a-carbinolamine dehydratase (dimerization cofactor of hepatic nuclear factor 1-alpha); Pterin carbinolamine dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha
• Gene ID: 5092
• mRNA Refseq: NM_000281
• Protein Refseq: NP_000272
• MIM: 126090
• UniProt ID: P61457
• Chromosome Location: 10q22
• Pathway: Metabolism of amino acids and derivatives
• Function: 4-alpha-hydroxytetrahydrobiopterin dehydratase activity; identical protein binding; lyase activity; phenylalanine 4-monooxygenase activity; protein binding; transcription coactivator activity