Recombinant Human PCK2 Protein, His/GST-tagged

• Cat.No.: PCK2-513H
• Product Overview: Recombinant Human PCK2 (NP_004554.3) (Leu33-Met640) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His/GST
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PCK2 consists of 845 amino acids and predicts a molecular mass of 94.9 kDa.
• Protein length: Leu33-Met640
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PCK2 phosphoenolpyruvate carboxykinase 2 (mitochondrial) [ Homo sapiens ]
• Official Symbol: PCK2
• Synonyms: PCK2; phosphoenolpyruvate carboxykinase 2 (mitochondrial); phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEPCK; PEPCK2; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase; PEPCK-M
• Gene ID: 5106
• mRNA Refseq: NM_001018073
• Protein Refseq: NP_001018083
• MIM: 614095
• UniProt ID: Q16822