Recombinant Human PCSK9 (D374Y) protein, His-tagged

• Cat.No.: PCSK9-2137H
• Product Overview: Recombinant Human PCSK9 (Gln31-Gln692, D374Y) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln31-Gln692
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).Especially,the D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Predicted N Terminal: Gln31
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: Human PCSK9 (D374Y) has a calculated MW of 72.0 kDa. The reducing (R) protein migrates as 17 kDa and 66 kDa in SDS-PAGE due to glycosylation and proteoly digestion. The D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PCSK9
• Official Symbol: PCSK9
• Synonyms: FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
• Gene ID: 353175
• mRNA Refseq: NM_174936.4
• Protein Refseq: NP_777596.2
• MIM: 607786
• UniProt ID: Q8NBP7