Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Gln31-Gln692 |
Tag : |
C-His |
Form : |
Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 10% glycerol (pH 7.4). |
Bio-activity : |
Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. |
Molecular Mass : |
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Storage : |
Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |