Recombinant Human PCSK9 (R194A) protein, His-tagged

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Recombinant Human PCSK9 (R194A) protein, His-tagged

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Cat.No. : PCSK9-289H
Product Overview : Recombinant Human PCSK9 (Gln31-Gln692, R194A) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln31
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : Human PCSK9 (R194A) has a calculated MW of 71.8 kDa. DTT-reduced Protein migrates as 17 kDa and 66 kDa in SDS-PAGE due to glycosylation and proteolytic digestion. The R194A mutation results in lower affinity of PCSK9 for LDLR.
Protein length : Gln31-Gln692
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of PCSK9-289H :

Human PCSK9 (R194A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of PCSK9-289H :

Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Human PCSK9 (R194A), His Tag with a linear range of 0.078-1.25 μg/mL (QC tested).

Gene Name : PCSK9
Official Symbol : PCSK9
Synonyms : FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
Gene ID : 353175
mRNA Refseq : NM_174936.4
Protein Refseq : NP_777596.2
MIM : 607786
UniProt ID : Q8NBP7

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