Recombinant Human PCSK9 (R194A) protein, His-tagged
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|Product Overview :||Recombinant Human PCSK9 (Gln31-Gln692, R194A) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).|
|Predicted N Terminal :||Gln31|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||Human PCSK9 (R194A) has a calculated MW of 71.8 kDa. DTT-reduced Protein migrates as 17 kDa and 66 kDa in SDS-PAGE due to glycosylation and proteolytic digestion. The R194A mutation results in lower affinity of PCSK9 for LDLR.|
|Protein length :||Gln31-Gln692|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of PCSK9-289H :||
Human PCSK9 (R194A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of PCSK9-289H :||
Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Human PCSK9 (R194A), His Tag with a linear range of 0.078-1.25 μg/mL (QC tested).
|Gene Name :||PCSK9|
|Official Symbol :||PCSK9|
|Synonyms :||FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3|
|Gene ID :||353175|
|mRNA Refseq :||NM_174936.4|
|Protein Refseq :||NP_777596.2|
|UniProt ID :||Q8NBP7|
◆ Recombinant Protein
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|Pcsk9-020M||Recombinant Mouse Pcsk9 Protein, MYC/DDK-tagged||+Inquiry|
|PCSK9-03H||Recombinant Human PCSK9 Protein, His-tagged||+Inquiry|
|PCSK9-692H||Recombinant Human PCSK9 Protein (Met1-Gln692), MIgG1 Fc-tagged||+Inquiry|
|PCSK9-2306RCL||Recombinant Rat PCSK9 cell lysate||+Inquiry|
|PCSK9-2875HCL||Recombinant Human PCSK9 cell lysate||+Inquiry|
|PCSK9-2564MCL||Recombinant Mouse PCSK9 cell lysate||+Inquiry|
◆ Assay kits
|Kit-1806||PCSK9(D374T)-LDLR TR-FRET Assay Kit||+Inquiry|
|Kit-1807||PCSK9[Biotinylated]-LDLR Binding Assay Kit||+Inquiry|