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Recombinant Human PDCD1, His-tagged

Cat.No. : PDCD1-217H
Product Overview : Recombinant Human PDCD1 /PD1 /CD279 MS Standard Protein, C13 and N15-labeled (PD1/PDCD1 (21-167), Heavy Labeled) Pro 21 - Gln 167 (Accession # NP_005009.2) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtai
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Description : Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Molecular Mass : PD1/PDCD1 (21-167), Heavy Labeled is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 17.3 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 24-45 kDa in SDS-PAGE due to glycosylation.
Endotoxin : Less than 1.0 EU per μg of the PD1/PDCD1 (21-167), Heavy Labeled by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution : See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name : PDCD1 programmed cell death 1 [ Homo sapiens ]
Official Symbol : PDCD1
Synonyms : PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l;
Gene ID : 5133
mRNA Refseq : NM_005018
Protein Refseq : NP_005009
MIM : 600244
UniProt ID : Q15116
Chromosome Location : 2q37.3
Pathway : Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem; T cell receptor signaling pathway, organism-specific biosystem;
Function : protein binding; protein tyrosine phosphatase activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (14)

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What are the mechanisms by which PDCD1 modulates the immune response to viral infections? 07/31/2022

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What are the mechanisms by which PDCD1 modulates the immune response to viral infections? 11/09/2021

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What role does PDCD1 play in the development of immune tolerance? 10/15/2021

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How does PDCD1 influence the balance between immune activation and autoimmunity? 05/23/2021

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer? 08/13/2020

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 influence the balance between immune activation and autoimmunity? 08/05/2020

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers? 12/05/2019

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer? 11/21/2019

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells? 04/24/2019

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers? 01/19/2019

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

What role does PDCD1 play in the development of immune tolerance? 12/08/2018

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression? 06/24/2018

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells? 06/24/2018

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression? 02/06/2018

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.

Customer Reviews (3)

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Reviews
06/06/2021

    Timely antibody validation, a valuable asset for our laboratory.

    02/12/2021

      Precise protein quantification, fundamental for maintaining data accuracy.

      06/20/2019

        Dependable peptide synthesis, accelerates our peptide-based projects effectively.

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