Recombinant Human PDCD1 protein, His-tagged (HPLC verified)

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Recombinant Human PDCD1 protein, His-tagged (HPLC verified)

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Cat.No. : PDCD1-332H
Product Overview : Recombinant Human PDCD1 (Leu25-Gln167) protein (HPLC verified) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Leu25
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 16.8 kDa. The protein migrates as 31-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Leu25-Gln167
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of PDCD1-332H :

Human PD-1, His Tag (HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC of PDCD1-332H :

The purity of Human PD-1, His Tag (HPLC verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA :

Immobilized Human PD-1, His Tag (HPLC verified) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL (Routinely tested).

Bioactivity-ELISA of PDCD1-332H :

Immobilized Human PD-1, His Tag (HPLC verified) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).

Bioactivity-SPR of PDCD1-332H :

Opdivo (Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag (HPLC verified) with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of PDCD1-332H :

Loaded Human PD-1, His Tag (HPLC verified) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC verified) with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Batch consistency of Bioactivity-BLI

Loaded Human PD-1, His Tag (HPLC verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Loaded Human PD-1, His Tag (HPLC verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Gene Name : PDCD1
Official Symbol : PDCD1
Synonyms : PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
Gene ID : 5133
mRNA Refseq : NM_005018
Protein Refseq : NP_005009
MIM : 600244
UniProt ID : Q15116

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