Recombinant Human PDCD1 protein, His-tagged, low endotoxin (HPLC-verified)
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|Product Overview :||Recombinant Human PDCD1 (Leu25-Gln167) protein (low endotoxin, HPLC-verified) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.|
|Predicted N Terminal :||Leu25|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||This protein has a calculated MW of 16.8 kDa. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation.|
|Protein length :||Leu25-Gln167|
|Endotoxin :||Less than 0.01 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of PDCD1-892HL :||
Human PD-1, His Tag, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|SEC-HPLC of PDCD1-892HL :||
The purity of Human PD-1, His Tag, low endotoxin (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
Immobilized Human PD-1, His Tag, low endotoxin (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL (Routinely tested).
|Bioactivity-BLI of PDCD1-892HL :||
Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC-verified) with an affinity constant of 23.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
|Gene Name :||PDCD1|
|Official Symbol :||PDCD1|
|Synonyms :||PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l|
|Gene ID :||5133|
|mRNA Refseq :||NM_005018|
|Protein Refseq :||NP_005009|
|UniProt ID :||Q15116|
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