Description : |
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
96 kDa |
AA Sequence : |
MATFISVQLKKTSEVDLAKPLVKFI QQTYPSGGEEQAQYCRAAEELSKLR RAAVGRPLDKHEGALETLLRYYDQI CSIEPKFPFSENQICLTFTWKDAFD KGSLFGGSVKLALASLGYEKSCVLF NCAALASQIAAEQNLDNDEGLKIAA KHYQFASGAFLHIKETVLSALSREP TVDISPDTVGTLSLIMLAQAQEVFF LKATRDKMKDAIIAKLANQAADYFG DAFKQCQYKDTLPKEVFPVLAAKHC IMQANAEYHQSILAKQQKKFGEEIA RLQHAAELIKTVASRYDEYVNVKDF SDKINRALAAAKKDNDFIYHDRVPD LKDLDPIGKATLVKSTPVNVPISQK FTDLFEKMVPVSVQQSLAAYNQRKA DLVNRSIAQMREATTLANGVLASLN LPAAIEDVSGDTVPQSILTKSRSVI EQGGIQTVDQLIKELPELLQRNREI LDESLRLLDEEEATDNDLRAKFKER WQRTPSNELYKPLRAEGTNFRTVLD KAVQADGQVKECYQSHRDTIVLLCK PEPELNAAIPSANPAKTMQGSEVVN VLKSLLSNLDEVKKEREGLENDLKS VNFDMTSKFLTALAQDGVINEEALS VTELDRVYGGLTTKVQESLKKQEGL LKNIQVSHQEFSKMKQSNNEANLRE EVLKNLATAYDNFVELVANLKEGTK FYNELTEILVRFQNKCSDIVFARKT ERDELLKDLQQSIAREPSAPSIPTP AYQSSPAGGHAPTPPTPAPRTMPPT KPQPPARPPPPVLPANRAPSATAPS PVGAGTAAPAPSQTPGSAPPPQAQG PPYPTYPGYPGYCQMPMPMGYNPYA YGQYNMPYPPVYHQSPGQAPYPGPQ QPSYPFPQPPQQSYYPQQTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |