Recombinant Human PDE12, GST-tagged

• Cat.No.: PDE12-1611H
• Product Overview: Recombinant full-length human PDE12 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full Length
• Description: PDE12 is 2', 5'-phosphodiesterase 12 protein that belongs to the CCR4/nocturin family. PDE12 cleaves the 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates. Triphosphorylated oligoadenylates, which are referred to as 2-5A, modulates in the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of PDE12 induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~100 kDa
• Purity: >90%
• Applications: PDE Assay
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDE12 phosphodiesterase 12 [ Homo sapiens ]
• Official Symbol: PDE12
• Synonyms: PDE12; phosphodiesterase 12; 2,5-phosphodiesterase 12; 2 PDE; 2 phosphodiesterase; DKFZp667B1218; 2-PDE; 2-phosphodiesterase; 2',5'-phosphodiesterase 12; 2'-phosphodiesterase; mitochondrial deadenylase; NP_808881.3; EC 3.1.13.4
• Gene ID: 201626
• mRNA Refseq: NM_177966
• Protein Refseq: NP_808881
• UniProt ID: Q6L8Q7
• Chromosome Location: 3p14.3
• Function: metal ion binding; poly(A)-specific ribonuclease activity