Recombinant Human PDE1A, His-tagged, Active

• Cat.No.: PDE1A-471H
• Product Overview: Recombinant full-length human PDE1A was expressed bybaculovirusinSf9 insect cellsusing an N-terminal His tag. MW=64kDa.

Specification

• Cat. No.: PDE1A-471H
• Description: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5"-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A. Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis. In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full length.
• Applications: PDE Assay.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• 22q13.33: 2q32.1

Gene Information

• Gene Name: PDE1A phosphodiesterase 1A, calmodulin-dependent [ Homo sapiens ]
• Synonyms: PDE1A; phosphodiesterase 1A, calmodulin-dependent; 3",5" cyclic nucleotide phosphodiesterase; OTTHUMP00000163419; OTTHUMP00000205290; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; calmodulin-dependent phosphodiesterase; phosphodiesterase 1A; phosphodiesterase-1A; HCAM1; HSPDE1A; MGC26303; EC 3.1.4.17
• Gene ID: 5136
• mRNA Refseq: NM_001003683
• Protein Refseq: NP_001003683
• MIM: 171890
• UniProt ID: P54750
• Pathway: Calcium signaling pathway; Progesterone-mediated oocyte maturation; Purine metabolism; Taste transduction; Opioid Signalling; Signaling by GPCR; Signalling by NGF
• Function: calmodulin binding; calmodulin-dependent cyclic-nucleotide; phosphodiesterase activity; hydrolase activity