Recombinant Human PDE4B protein, His & GST-tagged

• Cat.No.: PDE4B-1534H
• Product Overview: Recombinant Human PDE4B protein(NP_001032416.1) (Met 1-Thr 564) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-564 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 10% gly, 0.5mM PMSF, pH 8.0.
• Molecular Mass: The recombinant human PDE4B/GST chimera consists of 801 amino acids and has a calculated molecular mass of 92.2 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDE4B phosphodiesterase 4B, cAMP-specific [ Homo sapiens ]
• Official Symbol: PDE4B
• Synonyms: PDE4B; phosphodiesterase 4B, cAMP-specific; DPDE4, phosphodiesterase 4B, cAMP specific (dunce (Drosophila) homolog phosphodiesterase E4), phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila); cAMP-specific 3,5-cyclic phosphodiesterase 4B; phosphodiesterase E4 dunce homolog (Drosophila); PDE32; dunce-like phosphodiesterase E4; cAMP-specific phosphodiesterase-4 B isoform; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); DPDE4; PDE4B5; PDEIVB; MGC126529; DKFZp686F2182
• Gene ID: 5142
• mRNA Refseq: NM_001037339
• Protein Refseq: NP_001032416
• MIM: 600127
• UniProt ID: Q07343