Recombinant Human PDE6G 293 Cell Lysate
Cat.No. : | PDE6G-3344HCL |
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Description : | Antigen standard for phosphodiesterase 6G, cGMP-specific, rod, gamma (PDE6G), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | PDE6G phosphodiesterase 6G, cGMP-specific, rod, gamma [ Homo sapiens ] |
Official Symbol : | PDE6G |
Synonyms : | PDE6G; phosphodiesterase 6G, cGMP-specific, rod, gamma; PDEG; retinal rod rhodopsin-sensitive cGMP 3,5-cyclic phosphodiesterase subunit gamma; rod cG-PDE G; GMP-PDE gamma; RP57; MGC125749; DKFZp686C0587; |
Gene ID : | 5148 |
mRNA Refseq : | NM_002602 |
Protein Refseq : | NP_002593 |
MIM : | 180073 |
UniProt ID : | P18545 |
Chromosome Location : | 17q21.1 |
Pathway : | Hemostasis, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Phototransduction, organism-specific biosystem; Phototransduction, conserved biosystem; Platelet homeostasis, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; |
Function : | 3,5-cyclic-GMP phosphodiesterase activity; cGMP binding; enzyme inhibitor activity; hydrolase activity; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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