Active Recombinant Human PDGFRA protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: PDGFRA-234H
• Product Overview: Active Recombinant Human PDGFRA protein(P16234)(Gln24-Glu524) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 24-524 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human PDGF R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PDGFRA platelet-derived growth factor receptor, alpha polypeptide [ Homo sapiens ]
• Official Symbol: PDGFRA
• Synonyms: PDGFRA; platelet-derived growth factor receptor, alpha polypeptide; platelet-derived growth factor receptor alpha; CD140a; PDGFR2; PDGFR-alpha; PDGF-R-alpha; CD140a antigen; PDGFRA/BCR fusion; CD140 antigen-like family member A; platelet-derived growth factor receptor 2; alpha-type platelet-derived growth factor receptor; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; CD140A; PDGFR-2; RHEPDGFRA; MGC74795
• Gene ID: 5156
• mRNA Refseq: NM_006206
• Protein Refseq: NP_006197
• MIM: 173490
• UniProt ID: P16234